Technology

Astrego’s technology for Antibiotic Susceptibility Testing (AST) is founded on microfluidics and image analysis methods developed for pioneering microbial research in the Elf Lab at Uppsala University, as described in Baltekin et al, PNAS 2017 (Antibiotic susceptibility testing in less than 30 min using direct single-cell imaging).

Advantages of the Astrego Technology

  • Phenotypic
  • Fastest possible
  • Ultimate sensitivity
  • Cost effective
  • Versatile

How does it work?

Step 1

The sample is loaded on the microfluidic chip. Bacteria present in the sample are caught in the bacteria-sized traps.

Bacteria cells enter the channels of the microfluid chip

Step 2

Trapping of bacteria is monitored and the loading time gives an estimate of the bacterial density in the sample.

A fraction of the trapped bacteria are exposed to a candidate antibiotic.

Half of the bacterias is exposed to antibiotics to determine antibiotic susceptibility

Step 3

Bacterial growth are monitored in each trap, both with and without candidate antibiotic.

Klebsiella growing in the channels of the microfluid chip

Step 4

The average growth rates, with and without candidate antibiotic, are calculated in real time. The bacteria is considered susceptible if its growth is duly inhibited.

The bacterias exposed to antibiotics show declining growth rate, indicating susceptibility

The qUTI system

To date Astrego has focused mainly on urinary tract infection (UTI). The qUTI system, demonstrated in the video below, will rapidly and reliably diagnose UTI and test for antibiotic susceptibility at the Point-of-Care.

Benefits

Healthcare System

A highly requested and currently unavailable diagnostic tool for evidence based management of infections.

Improved monitoring of local, regional or global antibiotic resistance.

Pharma Industry

Extend life-time of primary antibiotics beyond 20% resistance.

Efficient tool for product development and clinical evaluation of new antibiotics.

Patient

Always correct diagnosis and immediate prescription of optimal antibiotic.

Reduced discomfort and less side effects from use of broad spectrum antibiotics.

Reduced risk of severe complications due to untreated infections.

Society

Accurate diagnosis of bacterial infection avoids misuse of antibiotics.

Reduces risk for antibiotic resistance development.

Fewer sick-days – lower cost